Protein Purification Reagents and Kits
Applied Biosystems™ S2B, Ethyl Acetate, 450 mL
This reagent is intended for N-terminal protein sequencing applications.
Bio Rad Laboratories POLYPRP COLMN 2ML 9CM LNG 50PK
Poly-Prep Columns, 50pk, Height 9cm, Hold up to 2mL of chromatography media and 10mL or eluent or sample in an integral reservoir, Includes end caps and tip closures
Anti-DYKDDDDK Affinity Resin, G-Biosciences
Anti‐DYKDDDDK Affinity Resin is suitable for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems. DYKDDDDK, commonly known as FLAG™ sequence (FLAG™ is a registered trademark of Sigma).
G-Biosciences DetergentOUT™ Tween™, Maxi
DetergentOUT™ Tween™, Maxi (10 mL) is used for fast and efficient removal of polysorbate or Tween™ detergents.
G-Biosciences DetergentOUT™ Tween™, Gravity Flow Column
Pre-packed, spin and gravity flow column format, detergent removal resin columns for polysorbate or Tween™ detergents or surfactants.
Zymo Research Corporation His-Spin Protein Miniprep™ Kit (50 Preps)
The simplified procedure is based on our innovative protein purification chemistry and custom designed fast spin columns. Up to 1 mg of His tagged protein can be purified in 5 minutes and eluted in as little as 100 ul of His-Elution Buffer. The purifiedprotein can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other applications. The product has been optimized for maximal protein purity: a single protein band is visible by Coomassie blue staining on SDS-PAGE gel. The straightforward spin wash elute protocol dramatically simplifies protein purification: get results in minutes, not hours.
STEMCELL Technologies EasySep™ Dead Cell Removal (Annexin V) Kit, For processing: 1 x 10^9 cells
The EasySep™ Dead Cell Removal (Annexin V) kit is designed to deplete apoptotic (Annexin V+) cells from cell culture or tissue preparations by immunomagnetic negative selection. Unwanted cells are labeled with Annexin V, antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are simply poured off into a new tube. Annexin V binds phosphatidylserine on the outer leaflet of the cell membrane during apoptosis.
STEMCELL Technologies EasySep™ Human Cord Blood CD34 Positive Selection Kit II, For processing: 1000 mL cord blood processed
Complete kit for CD34+ cells from cord blood
STEMCELL Technologies EasySep™ Human CD4+ T Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD4+ T Cell Enrichment Kit is designed to isolate CD4+ T cells from fresh or previously frozen peripheral mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD4+ T cells and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For even faster cell isolations, we recommend the new EasySep™ Human CD4+ T Cell Isolation Kit (17952) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-CD4-T-Cell-Isolation-Kit.aspx) which isolates cells in just 8 minutes.
STEMCELL Technologies EasySep™ Human CD4 Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Human CD4 Positive Selection Kit II is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection. Desired cells are targeted with antibody complexes recognizing CD4 and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Human CD4 Positive Selection Kit (Catalog #18052) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human CD34 Positive Selection Kit II, For processing: 5 x 10^9 cells
The EasySep™ Human CD34 Positive Selection Kit II is designed to isolate CD34+ cells from fresh or previously frozen bone marrow or mobilized peripheral blood mononuclear cells, or from previously frozen cord blood mononuclear cells, or from hESC/hiPSC-derived hematopoietic cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD34 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD34 antigen is expressed on hematopoietic stem and progenitor cells.This product can be used in place of the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human CD45 Depletion Kit II, For processing: 2 x 10^9 cells
The EasySep™ Human CD45 Depletion Kit II is designed to deplete CD45+ cells from fresh or previously frozen human peripheral blood mononuclear cells. Unwanted cells are targeted with antibody complexes recognizing CD45 and magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off. The CD45 antigen is expressed on all hematopoietic cells except erythrocytes and platelets.This product replaces the EasySep™ Human CD45 Depletion Kit (Catalog #18259) for even faster cell isolations.
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD19 Positive Selection Kit is designed to isolate CD19+ cells from whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD19 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD19 antigen is present on mature B cells and B cell progenitors but is lost upon maturation to plasma cells. It is also expressed on follicular dendritic cells.This product replaces the EasySep™ Human Whole Blood CD19 Positive Selection Kit (Catalog #18084) for even faster cell isolations.